Meta-Analysis Raw Data

This PRIVATE area of the Nzitrap.com web site can only be accessed from the link on the Research Home page. It contains the raw data and analyses supporting a meta-analysis of Nzi trap performance for standardized experiments conducted with numerous collaborators, mostly from the 1990's. This is still a "WORK IN PROGRESS", but so far all of the comparative, and standardized experimental data from Mihok and collaborators have been summarized.

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Fabric Resources Meta--Analysis

 

bulletEach experiment is summarized in a WORD file.

Each file is a straightforward guide that includes ANOVA output generated by SYSTAT. Some of these files are brief as I have not taken the time to annotate everything in detail. The file name corresponds to the experimental code in the EXCEL reference NziTrials. Raw data for three recent experiments are consolidated in another small file: Recent Trials. Original data are missing for one experiment (BERNDE02).
 

bulletThe RAW data and detailed statistics are in an EXCEL file.

Each file contains a RAW worksheet for one experiment extracted from the master reference and crosschecked against original records. Log (X+1) transformed catch data are calculated in a LOG worksheet via the macro CTRL+C using formulas in the TEMP worksheet. Note that you will get a macro warning when you open these EXCEL files.

The LOG file was imported into SYSTAT for statistical calculations. SYSTAT was used to provide the Mean Square Error, Degrees of Freedom and sample size information required to estimate response ratios. The CATCHES worksheet provides a quick point of reference for the species captured. These files may contain short notes on species or missing values but in general follow the same format throughout. Relevant response ratio data were transferred to the master database of Response Ratios using the worksheet DBASE.

 
bulletThe reflectance (cloth) and transmittance (netting) data are in a second EXCEL file.

These files were created several years ago and are a quick source of information for the fabrics used to make traps. They should contain the same information as the master fabric references in EXCEL and in ACCESS. I have not taken the time to confirm this.
 
bulletThe SYSTAT data file created from the log (X+1) worksheet in the EXCEL data file is provided along with a text file of the SYSTAT commands used to generate the ANOVAS.

These files can be used with SYSTAT (or any similar statistical package with minor adjustments) to analyze the data with a simple modification of the directory references at the beginning of the command file. Note that these files may contain commands specific to idiosyncrasies of each experiment. Note that the RAW and LOG worksheets in the original EXCEL file contain the same header row. Hence, to analyze untransformed data, simply import the RAW worksheet into SYSTAT and re-analzye the data with the command file.
 
bulletCodes for other standard biting fly traps should be self-evident. Some files will contain additional experimental Nzi traps with codes that are meaningful only to me until this work is formally published.

All key trials include a NZI-COT standard trap (phthalogen blue cotton, Ethiopian or Kenyan fabric), and most also include a NZI-POL standard trap (Vestergaard Frandsen's original "pongee 2" pure blue 100% polyester from the mid-1990's). The original goal of this research was to test Nzi traps made from blue fabrics #105 and #101 in 3-times replicated 3 x 3 Latin squares relative to a "local" standard trap. Fabrics #105 and #101 are simply large lots of the equivalent fabrics #60 and #65 that did well when tested in Expt 23 of Mihok (2002).

 

Country Area Season Summary Data File Commands Catches Fabrics
      WORD SYSTAT TEXT EXCEL EXCEL
3 x 3 Latin Squares - Ethiopian Phthalogen Blue Cotton #105 & Vestergaard Frandsen Polyester #101
Kenya Nguruman Wet TrNguWet NguWet 6k 2,930k 310k
Kenya Kibwezi Wet TrKib Kib 5k 2,644k 308k
Kenya Mbita Point Wet TrMbita Mbita 3k 2,112k 308k
Ethiopia Didesa Wet TrDidesa Didesa 6k 3,305k 308k
Ethiopia Arba Minch Wet TrMinchWet MinchWet 6k 2,942k 308k
Tanzania Naitolya Dry TrTPRI TPRI 2k 2,151k 240k
Uganda Busoga Dry TrUganda Uganda 3k 2,145k 274k
Zambia Kafue Wet TrZambWet ZambWet 3k 2,167k 308k
Zambia Kafue Dry TrZambDry ZambDry 3k 2,167k 308k
Gambia Niamina East Wet TrGambia Gambia 4k 2,476k 340k
Denmark Copenhagen Wet TrDenmark Denmark 2k 1,936k 206k
USA* Iowa Dry TrIowa Iowa 3k 1,824k 307k
USA* Louisiana Dry TrLouisiana Louisiana 2k 1,965k 275k
Japan Hokkaido Dry TrJapan Japan 1k 1,871k 173k

* Brief results published in:  Mihok, S., Carlson, D.A., Krafsur, E.S. & Foil, L.D. (2006) Performance of the Nzi and other traps for biting flies in North America. Bulletin of Entomological Research, 96, 387-397.

Minor Methodological Variations - Cotton and polyester both tested
Phthalogen Blue Cotton from Mountex, Kenya #184 & Opaque VF Polyester #161
Ethiopia Kombo Wet TrKombo Kombo 5k 2,578k 274k
Mozambique Quelimane Dry TrMozam Mozam 2k 2,182k 308k
Phthalogen Blue Cotton from Bonar Industries, Zimbabwe #171
Cotton trap with geometric frame, Standard VF Polyester #101
Zimbabwe Zambezi Wet TrZimbWet ZimbWet 2k 2,301k 240k
ROYAL BLUE Cotton from Awassa Textiles, Ethiopia #127, Standard VF  Polyester #101
Mali Tienfala-Baguineda Wet TrMaliWet MaliWet 3k 2,511k 239k
Mali Madina-Diassa
(Savanna)
Dry TrMaliDryA MaliDryA 2k 2,264k As Above
               
Complex Fabric Trials - Large Latin Squares with many special fabrics
Experiment 23 from Mihok (2002) in Bulletin of  Entomological Research
Ethiopian #60, Kenyan #62 Phthalogen Blue & VF Polyesters #64, 65 and some size/netting variations
Kenya Nguruman Dry TrNguDry NguDry 5k 4,093k 343k
Initial fabric trial to scope causes of polyester/cotton catch differences - various cotton/polyester fabrics
ROYAL BLUE Cotton from Awassa Textiles, Ethiopia #127 tested
Ethiopia Arba Minch Dry TrMinchDry MinchDry 3k 2,662k 587k
Three sequential experiments using the same set of traps in minor variations on cotton/polyester fabrics
Comparing many possible sources of catch differences in fabric features
Ethiopia Chanka1 Wet TrChanka1 Chanka1 4k 3,630k 773k
Ethiopia Chanka2 Wet TrChanka2 Chanka2 6K 4,680k As Above
Kenya Shimba Wet TrShimba Shimba 4k 3,731k 773k
               
Various Experiments - ONLY Cotton Nzi traps being compared to other traps
Mihok, S., Carlson, D.A. & Ndegwa, P.N. (2007) Tsetse and other biting fly responses to Nzi traps baited with octenol, phenols and acetone. Medical and Veterinary Entomology, (in press).
 7 x 7 Latin Squares -
Phthalogen Blue
Cotton from Mountex, Kenya #184
Ethiopia Chanka Wet TrTrapEth TrapEth 6k 3,978k 137k
Phthalogen Blue Cotton from Bonar Industries, Zimbabwe #171
Tested dark versus light netting - traps made with geometric frames
Zimbabwe Zambezi Dry TrZimDry ZimDry 3k 2,324k 240k
Standard Phthalogen Blue Cotton from Awassa Textiles, Ethiopia #105
Burkina Faso Lahirasso Dry TrBFLah BFLah 3k 2,437k 171k
Burkina Faso Kimini Dry TRBFKimini BFKimini 2k 2,488k As Above
Burkina Faso Banwali Dry TrBFBanwali BFBanwali 2k 2,340k As Above

Published Trials using the NZI-COT trap
Lighter-weight, Phthalogen Blue "Jinja" cotton from Mountex, Kenya #27

Details Area Season Summary Data File Commands Catches Fabrics
      WORD SYSTAT TEXT EXCEL EXCEL
TANZANIA - Ndegwa, P.N. & Mihok, S. (1999) Development of odour-baited traps for Glossina swynnertoni Austen (Diptera: Glossindae). Bulletin of Entomological Research, 89, 255-261.

Original data were only saved from Expt #3; raw ANOVA output  from author used for expt #2

Expt 2 Naitolya Dry TRBerNde02 BerNde02
(EXCEL)
No Raw Data 240k
Expt 3 Naitolya Dry TRBerNde03 BerNde03 3k 2,276k As above
KENYA -  Mihok, S. (2002) The development of a multipurpose trap (the Nzi) for tsetse and other biting flies. Bulletin of Entomological Research, 92, 385-403.

Major 8x8 trial of 3 colour options against multiple trap standards, G. pallidipes data analyzed for 2 replicates

Expt 12 Nguruman Dry TrBerMih12
 
BerMih12
GP Subset
4k 4,249k
GPdata
240k

Three colour options against Vavoua for Stomoxys only

Expt 13 Nairobi Dry TrBerMih13 BerMih13 4k 2,433k 240k

Major trial, Nzi and Red Nzi against Vavoua trap for Stomoxyinae only - forest, high density

Expt 14 Nairobi Park Dry TrBerMih14 BerMih14 6k 3,108k 240k

Only  test of very first Vestergaard Frandsen Polyester "Brilliant Blue 589"

Expt 15 Nairobi Dry TrBerMih15 BerMih15 4k 2,310k 308k

Three colour options against Vavoua for Glossina longipennis, Philoliche

Expt 16 Maralal Dry TrBerMih16 BerMih16 3k 2,504k 240k
Exploratory trap variations with data on Canopy trap - NOTE small sample sizes
Expt 17 Nguruman Dry TrBerMih17 BerMih17 5k 2,249k 240k
Many Trap variations with good data for Tabanidae, including Canopy trap
Expt 18 Ruma Park Dry TrBerMih18 BerMih18 6k 4,385k 240k
Trap variations as in #18 with data on Glossina fuscipes
Expt 19 Mbita Point Dry TrBerMih19 BerMih19 6k 3,795k 240k
Major trial of early VF polyesters and comparisons to multiple standard traps
Expt 20 Shimba Hills Wet TrBerMih20 BerMih20 5k 4,275k 378k
Phthalogen Blue Cotton from Mountex, Kenya #184, Recent Experiments in North America with EXCEL files in different format; raw data stored in a separate database in ACCESS
CANADA  Mihok, S., Carlson, D.A., Krafsur, E.S. & Foil, L.D. (2006) Performance of the Nzi and other traps for biting flies in North America. Bulletin of Entomological Research, 96, 387-397.

Selected comparisons to a few other biting fly traps in experiments conducted for various purposes

Vavoua Russell, ON Sep 2003 Ber06Net2 Net2 2k 925k  
Alsynite Russell, ON Sep 2004 Ber06Dyes5 Dyes5 2k 1,021k  
Canopy Russell, ON Jul-Aug 2004 Ber06Canopy Canopy 2k 982k  
Further original data are available on request for the recent experiments described in this publication

Miscellaneous Nzi Data NOT Amenable to Trap & Fabric Meta-Analysis
Experimental traps/odours only, and/or data idiosyncrasies

Details Area Season Summary Data File Commands Catches Fabrics
      WORD SYSTAT TEXT EXCEL EXCEL
Phthalogen Blue Cotton from Mountex, Kenya #184
Mihok, S., Carlson, D.A. & Ndegwa, P.N. (2007) Tsetse and other biting fly responses to Nzi traps baited with octenol, phenols and acetone. Medical and Veterinary Entomology, (in press).
 8 x 8 Latin Squares Acetone - Octenol - Cattle Urine Baits using NZI-COT traps
Ethiopia Chanka Dry TrBaitEth BaitEth 6k 2,486k 137k

Mixed  Odour & Bait Tests - losses of several  traps due to fires set by farmers

Ethiopia Chanka Dry       In Prep 137k
KENYA -  Mihok, S. (2002) The development of a multipurpose trap (the Nzi) for tsetse and other biting flies. Bulletin of Entomological Research, 92, 385-403.
Lighter-weight, Phthalogen Blue "Jinja" cotton from Mountex, Kenya #27

First ever test of the final format of the NZI-COT trap, but with a black inner shelf instead of a netting shelf

Expt 10 Nguruman Wet In Prep 240k

Variations on inner shelves and baffles - only NZI-COT trap used

Expt 11 Nguruman Wet In Prep 240k
Test for Stomoxys of unattractive outer surfaces - only NZI-COT trap used
Expt 21 Nairobi Wet In Prep 240k
Test for tsetse of unattractive outer surfaces, trap floor, etc. - only NZI-COT trap used
Expt 22 Nguruman Dry In Prep 240k
MALI - ROYAL BLUE Cotton from Awassa Textiles, Ethiopia #127, Standard VF  Polyester #101
Catches too low for useful statistics
Mali Madina-Diassa Dry TrMaliDryB MaliDryB 2k 2,045k 239k

Updated
12-Aug-2012